RNA
Part:BBa_K4950003:Design
Designed by: Lucas Ng Group: iGEM23_PennState (2023-10-12)
mCRP2 Riboswitch Insert
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Previously published apatamer sequence was required along with its known binding affinity [1]. Design was selected for its high predicted activation ratio and distinctness from other designs.
Source
Designed by De Novo DNA Riboswitch Calculator
References
[1] Bini, A., Centi, S., Tombelli, S., Minunni, M., & Mascini, M. (2008). Development of an optical RNA-based aptasensor for C-reactive protein. Analytical and bioanalytical chemistry, 390(4), 1077–1086. https://doi.org/10.1007/s00216-007-1736-7